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Mycoplasma Pneumoniae IgM Test Kit(Colloidal Gold)

 The Diagnostic Automation Mycoplasma Pneumonia IgM kit is intended for the qualitative determination of IgM antibodies in human serum to Mycoplasma pneumonia antigen. 

The DAI anti- Mycoplasma IgM assay may be used as an aid in the diagnosis of Mycoplasma pneumoniae infection in the adult population.


Materials Provided with Mycoplasma Pneumonia IgM Test  Kit:

1. Microassay plate:wells coated with inactivated M. pneumonia (strain FH). antigen

2. HRP Conjugate: Ready to use, Goat anti-human IgM

3. Positive Control: Human serum

4. Calibrator: Human serum

5. Negative Control: Human serum

6. Serum Diluent Type I, Ready to use

7. TMB, Ready to use

8. Stop Solution: Ready to use,1N H2SO4 solution

9. Wash Buffer 10X


Materials required but not provided:

1. Freshly distilled or deionized water

2. Dispensing system and/or pipette

3. kit Microplate washer

4.  kit Microplate Reader with 450 single & 600-650nm dual wavelength


Mycoplasma Pneumonia IgM Test Kit Background Information:

Mycoplasma pneumoniae is among the smallest of free-living organisms. In humans, it is usually found in the throat and lungs and is susceptible to antimicrobial agents that inhibit protein synthesis. The spread of mycoplasmal infections depends on close and prolonged contact between people, as it is transmitted by means of droplet nuclei from the respiratory tract. Mycoplasmal infection may be asymptomatic or may produce upper respiratory tract disease or atypical pneumonia. The pneumonia is difficult to differentiate from viral diseases by clinical means alone Laboratory tests such as isolation, Complement Fixation serology, and ELISA serology are helpful as an aid in the diagnosis.


Mycoplasma Pneumonia IgM Test Principle:

When antigens bound to the solid phase are brought into contact with a patient serum, antigen specific antibody, if present, will bind to the antigen on the solid phase forming antigen- antibody complexes. Excess antibody is removed by washing. This is by the addition of goat anti-human IgM globulin conjugated with horseradish peroxidase which then binds to the antibody-antigen complexes. The excess conjugate is removed by washing, by the addition of substrate and chromogen. If specific antibody to the antigen is present in the serum, a blue color develops which is proportional to the concentration of antibody in the serum.

For more information about  Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.


Product Note:

Each time DAI Mycoplasma pneumonia IgM assay is run, the Calibrator must be run in triplicate. A reagent blank, Negative Control, and Positive Control must also be included in each assay. Calculate the mean of the three Calibrator wells. If any of the three values differ by more than 15 percent from the mean, discard that value and calculate the mean using the remaining two wells. The mean OD value for the Calibrator and the OD values for the Positive and Negative Controls should fall within the expected ranges.

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