Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) 1. Product Information Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) provides a fast measurement for endotoxin quantification. The endotoxin in the sample activates a cascade of enzymes in Amebocyte Lysate, the activated enzyme splits the synthetic substrate, releasing a yellow-colored item. Then the yellow item can further react with diazo reagents to form purple items with maximum absorbance at 545nm. A regular spectrophotometer or plate reader is required for the assay. The purple items are proportional to endotoxin concentration. Then endotoxin is quantified. 2. Product Parameter Sensitivity Range: 0.01-0.1EU/ml (assay time about 46 minutes) 0.1-1.0EU/ml (assay time about 16 minutes) 3. Product Features and Application Bioendo EC Endotoxin Test Kit (End-...
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