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  We have developed a practical procedure for expedient and cost-effective synthesis of small to medium-sized membrane proteins in multi-milligram scale through the use of automated Fmoc chemistry. After the attachment of a removable arginine-tagged backbone modification group, the membrane protein segments behave almost the same as ordinary water-soluble peptides in terms of Fmoc solid-phase synthesis, ligation, purification, and mass spectrometry characterization. The efficiency and practicality of the method is demonstrated by the successful preparation of Ser64-phosphorylated M2 proton channel from influenza A virus and the membrane-embedded domain of an inward rectifier K+ channel protein Kir5.1.  Figure1. Removable Arg-tagged backbone modification for chemical synthesis of membrane proteins Reference: ------------------------------------------------------------------------------------ 1.Zheng, J. S. et al. Expedient total synthesis of small to medium-sized membrane prot...

  Quality of fresh meat is closely linked with human’s health. Bacterial contamination and freshness of meat are the key factors to show such quality. But TVB_N detection takes long time about 48 hours to get results, and sensory evaluation is not reliable sometimes. A rapid and accurate method to detect is necessary. TAL reagent  could be applied to detect bacterial contamination and freshness of meat. Because the main bacterial contamination of meat is gram-negative bacteria, which releases endotoxins when it dies. And endotoxins could be detected or quantified by TAL reagent. We will briefly describe how to check bacterial contamination and freshness of meat through TAL reagent here. Materials and Methods 1.BIOENDO TAL reagent. Test for confirmation of labeled lysate sensitivity should be carried out when a new batch of lysate is used or when there is any change in the test conditions that may affect the outcome of the test. 2. BIOENDO Water for BET  and diluent. Water...

In 2018,  SAT NANO  launched a special spherical powder for refractory metal  3D printing . Recently, SATNANO used its independent research and development intellectual property rights to provide customers with customized spherical tungsten powder, and tried to print tungsten series shielding parts. The whole series of parts adopt a thin-wall structure and the minimum wall thickness is only 0.1mm. As we all know, tungsten shields have good radiation shielding performance and use performance, and are widely used in PET rooms, linear accelerators, HDR rooms, gamma knives and nuclear power plants. Compared with traditional shielding materials, tungsten has higher density and better ray absorption ability, which can greatly reduce the volume of the shielding without weakening its shielding performance; in addition, tungsten shields are non-toxic and will not cause harm to the human body Hazard; and the purity of the formed parts reaches 99.9%, and the thermal conductivity abo...